Methodical course on laboratory immunetechniques in molecular biology research was organized by the University of Pécs, Medical School, Clinical Centre, Department of Immunology and Biotechnology between 11 – 15 December 2017. (Course code: OPEL_B-139_NEP1) Selected topics were organized in work packages. Theoretical lectures held by professionals of the Department introduced each work package and followed in own-hand laboratory trainings assisted by experts of the Department. The examination was performed in written test form. The accomplishment of course was equal to 6 ECTS credits.
Laboratory immunetechniques in molecular biology research
PhD course schedule
Course code: OPEL_B-139_NEP1
December 11-15, 2017.
December 11, Monday
8.30-10.00
Introduction.
Monoclonal and polyclonal antibodies.
Immunization, hybridoma technique.
Antibody purification and storage.
Antibody labelling for practical applications.
Lecturer: Prof. Dr. Péter Németh
10.30-12.00
Immunocytochemistry, immunohistochemistry, fluorescent microscopy, confocal microscopy.
Lecturer: Prof. Dr. Péter Balogh
14.00-16.00
Immunohistochemistry practice.
Laboratory practice: Prof. Dr. Péter Balogh
December 12, Tuesday
8.30-10.00
Haemagglutination, Coombs-test.
Nephelometry, turbidimetry.
ELISA, RIA.
Routine diagnostics, automatization.
Lecturer: Prof. Dr. Tímea Berki
10.30-12.00
Immunodiffusion, immunoelectrophoresis.
Dot blot, Westen blot.
Immunoprecipitation.
Lecturer: Dr. Péter Engelmann
14.00-16.00
ELISA routine diagnostic practice.
Laboratory practice: Zsuzsa Csizmadia
December 13, Wednesday
10.30-12.00
Molecular DNA techniques and their immunologic applications I.
Phage display and its applications in immunology.
Lecturer: Dr. Ferenc Boldizsár
14.00-16.00
Molecular biology practice.
Laboratory practice: Dr. Erzsébet Gajdócsi
December 14, Thursday
8.30-10.00
Significance of cell surface CD markers. Flow cytometry.
Cell surface and intracellular staining for flow cytometry.
Lecturer: Dr. Péter Engelmann
10.30-12.00
New possibilities in flow cytometry.
CBA technique.
Lecturer: Dr. Ferenc Boldizsár
14.00-16.00
Flow cytometry practice.
Laboratory practice: Réka Kugyelka
December 15, Friday
8.30-10.00
Molecular DNA techniques and their immunologic applications II.
Lentiviral vectors.
Lecturer: Dr. Ferenc Boldizsár
10.00-12.00
Consultation
14.00-16.00
Exam